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This bret signal can be detected by using the deep blue coelenterazine (dbc) substrate for rluc.
Bret 6 exhibited a bret efficiency of 83% with the shortest linker (fl 1), which decreased to 69% when the bret components were separated by the fl 9 linker (table s1).
These systems have revolutionized the way we. Bret relies on forster resonance energy transfer from a. Previously described bret systems include bret 1 and bret 2, which use renilla luciferase (rluc) as bret donors [4]. Here, we describe how recent improvements in detection devices and bret probes can be used to markedly improve the resolution of bret imaging, thus widening the.
